Green Silver Nanoparticles From 10 Plants Essay - 918 Words

Biofabrication of green silver nanoparticles from 10 plants; an efficient approach to inhibit multi-drug resistant microbes and their biofilms. Shariq Qayyuma and Asad U Khana a Medical microbiology and molecular biology, Laboratory Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India Author for correspondence: Email: asadukhan72@gmail.com Phone No.:+91- 9837021912 Abstract: Silver nanoparticles were biosynthesized via a green route using 10 different plants extracts and formed AgNPs were tested against drug resistant microbes and their biofilms. These nanoparticles were characterized using UV- vis spectroscopy and TEM which confirmed their synthesis, shape and size. FTIR clearly demonstrated the presence of the bio-groups on the surface of AgNPs. XRD confirmed the crystalline structure of AgNPs. TEM images were further analyzed using Image J software which showed that majority of particles were under 100 nm and majorly distributed over 1 to 60 nm size range. Their antimicrobial efficacy was checked against bacteria harboring antibiotic resistance genes like CTX-M-3, CTX-M-15, OXA-1, arm A, SHV-1 and NDM-1 in gram negative bacteria and fluconazole, amphotericin B and itraconazole resistant genes in fungus. Gram positive bacteria and fungi Candida albicans were inhibited at higher MICs values in comparison to Gran negative bacterial strains. 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